mgcl 2 solution (New England Biolabs)

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New England Biolabs mgcl 2 solution
Mgcl 2 Solution, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 564 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mgcl+2+solution/Magnesium+Chloride+(MgCl2)+Solution/pmc12922755-254-55-58
Average 96 stars, based on 564 article reviews

mgcl 2 solution - by Bioz Stars, 2026-09

96/100 stars

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Amplification:

Article Title: Detection of low-frequency DNA variants by targeted sequencing of the Watson and Crick strands
Article Snippet: .. If 11 cycles were used, the libraries were divided into eight aliquots and amplified in eight 50-μl reactions, each supplemented with an additional 0.5 U of Q5 Hot Start High-Fidelity DNA Polymerase (New England BioLabs, M0493L), 1 μl of 10 mM dNTPs (New England BioLabs, N0447L) and 0.4 μl of 25 mM MgCl 2 solution (New England BioLabs, B9021S). .. The products were purified with 1.8× SPRI beads (Beckman Coulter, {"type":"entrez-nucleotide","attrs":{"text":"B23317","term_id":"2508948","term_text":"B23317"}} B23317 ) and eluted in EB buffer (Qiagen).

Article Title: Evidence that extra copies of chromosome 1q play a role in the early phases of pancreatic neoplasia
Article Snippet: .. If 11 cycles were used, the libraries were divided into eight aliquots and amplified in eight 50-μl reactions, each supplemented with an additional 0.5 U of Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs, M0493L), 1 μl of 10 mM deoxynucleoside triphosphates (Deoxynucleoside triphosphates; New England Biolabs, N0447L) and 0.4 μl of 25 mM MgCl 2 solution (New England Biolabs, B9021S). .. The products were purified with 1.8× SPRI beads (Beckman Coulter, B23317 ) and eluted in EB buffer (Qiagen).

Incubation:

Article Title: A Hydrogel Sensing Pathological Urate Concentrations.
Article Snippet: Metabolite-responsive hydrogels that detect pathological metabolite concentrations and react by releasing a therapeutic stimulus hold high promises in treating metabolic diseases.. In this study, a hydrogel is described that discriminates between physiological and pathological concentrations of urate, the causative agent of gouty arthritis.. The hydrogel is synthesized by coupling a dimeric variant of the Deinococcus radiodurans -derived urate repressor HucR to linear polyacrylamide.

Binding Assay:

Polymerase Chain Reaction:

Article Title: New Approaches for Enhanced Detection of Enteroviruses from Hawaiian Environmental Waters
Article Snippet: .. Five microliters of cDNA was added to 20 μL PCR mix containing 1X Taq reaction buffer (NEB, MA), 2.0 mM MgCl 2 solution (NEB, MA), 200 nM of each dNTP (Sigma-Adrich, MO), 400 nM of forward and reverse primers (Integrated DNA Technologies, IA), and 2 units of Taq DNA polymerase (provided by Dr. Tung Hoang, University of Hawaii at Manoa). .. Reaction tubes were placed in a Mastercycler® Gradient (Eppendorf, Germany) for an initial denaturation at 94°C for 5 min., followed by 40 cycles of denaturation at 94°C for 30 sec., annealing at 56°C for 20 sec., and extension at 72°C for 30 sec., completed by a final extension at 72°C for 5 min. EnV detection was analyzed by gel electrophoresis.

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